《植物生理学报》 2009, 45(10): 986-990

生长素、乙烯和一氧化氮对拟南芥下胚轴插条形成不定根的调节

王金祥*, 陈碧丽, 廖红*, 严小龙

华南农业大学资源与环境学院, 根系生物学研究中心, 广州510642

通信作者：王金祥,廖红；E-mail: jinxwang@scau.edu, cnhliao@scau.edu.cn；Tel: 020-85280156

摘　要：

研究生长素、乙烯和一氧化氮(NO)对拟南芥下胚轴插条形成不定根的调节, 以及生长素和乙烯信号转导成员在IAA 促进不定根形成中的作用的结果表明: 拟南芥切条以IAA和硝普钠(NO 供体)单独处理7 d后的不定根形成均受到促进, 其 中以50 μmol•L-1 IAA和1 μmol•L-1 SNP的促进作用为最强, 乙烯的促进作用不明显; 生长素运输和信号转导以及乙烯信号转 导相关突变体对 IAA 促进生根作用的敏感性比野生型有所下降, 特别是 IAA14 功能获得型的突变体。IAA 和 NO 在促进不 定根形成中有协同效应。

关键词：拟南芥不定根; 下胚轴插条; 生长素; 乙烯; 一氧化氮

收稿：2009-08-15   修定：2009-09-10

资助：广东省自然科学基金(04300588)、教育部博士点基金(20040564004)和国家自然科学基金(30600380)。

Regulation of Auxin, Ethylene and Nitric Oxide on Adventitious Rooting in Arabidopsis Hypocotyl Cuttings

WANG Jin-Xiang*, CHEN Bi-Li, LIAO Hong*, YAN Xiao-Long

Root Biology Center, College of Natural Resources and Environment Sciences, South China Agricultural University, Guangzhou 510642, China

Corresponding author: WANG Jin-Xiang; E-mail: jinxwang@scau.edu, cnhliao@scau.edu.cn; Tel: 020-85280156

Abstract:

Arabidopsis hypocotyl cuttings were employed to study effects of auxin, ethylene and nitric oxide (NO) on adventitious rooting. The functions of auxin and ethylene signaling players cooperated with indole acetic acid (IAA) in adventitious rooting were also investigated. The results showed that treatment with either IAA or sodium nitroprusside (SNP, donor of NO) on Arabidopsis cuttings for 7 days could stimulate adventitious root formation, and the optimal concentration is 50 μmol·L^-1 and 1 μmol·L^-1, respectively; while ethylene played little role in rooting. Compared with wild type, sensitivity of auxin transportation and signal transduction and ethylene signaling-related mutants to stimulatory effect of IAA on rooting was reduced, especially gain-offunction of mutated IAA14 (SLR). NO had synergistic role with IAA in adventitious rooting.

Key words: Arabidopsis adventitious root; hypocotyl cutting; auxin; ethylene; nitric oxide